The quoted permissible RCF values (identical to g values) refer to filling with water and 20 min centrifugation time at 20°C. However, the actual stress limit is affected to a large extent by conditions such as the positioning in the rotor, chemicals, temperature and running time. There- fore, the RCF values must only be understood as a general guide. (DIN 58970).
Equation to calculate RCF:
RCF = 1,118 x r x (n/1000)2
Example:
Rotation radius r = 180 mm
(vertical distance between center of rotation axis and bottom end of centrifuge tube)
Speed n = 6000 min-1 (rotations per minute divided by 1000)
RCF = 1,118 x 180 x (6000/1000)2
= 7245
INTERNET USERS ALSO CONSULTED ON THE CATEGORY PLASTIC TUBES
See also